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Oil Sands Environmental Management Bibliography

The Cumulative Environmental Management Association (CEMA)partnered with the Oil Sands Research and Information Network (OSRIN) to create the new Oil Sands Environmental Management Bibliography, which includes documents relevant to the environmental management of oil sands development in Alberta. The majority of the documents focus on the mineable oil sands in the Athabasca deposit, though some documents relate to in-situ developments. This bibliography was last updated in November 2014.

The effects of an experimental spillage of oil sands tailings sludge on benthic invertebrates

Year of Publication: 1979

Abstract:
A very minor, instantaneous spillage of oil sands tailings sludge was introduced experimentally to a small part of a river in northern Alberta, Canada. A 60% reduction in the standing stock of benthic invertebrates occurred throughout a four-week period in the immediate area of the spillage. Sensitive indicator organisms decreased in abundance over an area of at least 30 m downstream of the spillage. The tailings sludge contained fine silt, heavy, sticky oils and heavy metals, all of which would have a deleterious effect on stream benthos. The fine silt mixed with the sticky oils of the sludge probably constituted the principal hazard to aquatic communities. It is recommended that appropriate measures should be established so as to prevent the addition of oil sands tailings sludge, by either accident or design, to lakes or rivers.

The effects of dimethylated and alkylated polycyclic aromatic hydrocarbons on the embryonic development of the Japanese medaka

Year of Publication: 2005

Abstract:
The Japanese medaka (Oryzias latipes) early-life stage assay was used to investigate the effects of a number of commercially available dimethylated polycyclic aromatic hydrocarbons (PAHs) (3,6-dimethylphenanthrene, 7,12-dimethylbenz[a]anthracene, and 4,6-dimethyldibenzothiophene) and their unsubstituted congeners, dimethylated and unsubstituted tertiary mixtures, and a complex environmental mixture (with elevated C2-substituted dibenzothiophene) on embryo larval development. Unsubstituted PAHs showed trends of increased blue sac disease (BSD) relative to dimethylated PAHs, although the severity of BSD induction varied. Results demonstrated that the dibenzothiophene congeners were the strongest inducers of BSD of the commercial PAHs tested. These compounds reduced the hatching success of embryonic medaka, an effect that was enhanced in the mixture. The base neutral extract significantly increased the frequency and severity of BSD abnormalities, while significantly reducing larval hatch length. Based on these results, a sublethal maximum allowable toxicant concentration (MATC) of 13.91 microg PAHs/L was calculated.

The effects of dual porosity on transport and retardation in peat: A laboratory experiment

Year of Publication: 2012

Abstract:
Peatlands cover up to half of the local landscape in the Athabasca oil sands region, and peat materials are commonly used in reclamation. The toxicity of oil sands process-affected water (OSPW) derived from the Athabasca oil sands is related to the elevated concentration of naphthenic acids (NAs) and sodium (Na). However, the transport and retardation of solutes through peat is significantly delayed by sorption and by diffusion into immobile water contained in the peat matrix. Approximately 94% of the 43.5 mg L−1 of OSPW was sorbed by 1 kg of peat. For Na ∼84% sorption occurred with 382 mg L−1 kg−1 of peat. The sorption and desorption of NAs and Na on peat fitted linear isotherms with distribution coefficients of 6.53 and 5.74 L kg−1, respectively. Solute breakthrough tests were performed for NaCl and the retardation due to sorption (R) of 1.73 was estimated for Na using a two-region (mobile and immobile) non-equilibrium transport model. However, the estimated mass transfer coefficient describing solute exchange between the mobile and immobile liquid regions indicated that part of retardation is attributed to solute exchange between the mobile and immobile phases. This was evident because Cl, a conservative solute (R=1, no sorption), also exhibited characteristics of dual-porosity transport. Thus, the passage of the solute front was retarded by diffusion of solute into the immobile region and by solute exchange between the mobile and immobile phases, which occurs at a rate depending on the proportion of mobile/immobile pore spaces. In this study, we showed that the complex dual-porosity structure of the peat is the important factor in attenuating solute transport where the presence of immobile phase (dead-end pores) in the system contributes to the transport and sorption mechanism of solute into this porous medium.

The effects of flocculant on the geotechnical properties of mature fine tailings: An experimental study

Year of Publication: 2012

Abstract:
When oil sands tailings are deposited in a tailing pond, they settle and segregate to create a layer of stagnant water on top that is reused in oil extraction and a dense mixture of clay, silt and water on the bottom which is referred to as mature fine tailings (MFT). Dewatering of MFT is a problem due to its very high fines content and relatively low solid content. Atmospheric drying offers a possible solution to this problem. In this technique, MFT are pumped or dredged out of the pond and mixed with polymers that helps to accelerate the release of water from the fines, and then placed on a sloped drying area in a thin layer. When the released water runs off the remaining flocculated MFT will dry to the desired moisture content for removal and replacement in the mine or for subsequent lifts. This paper presents a laboratory study on the flocculated MFT. With a senes of tests the basic properties and consolidation and desiccation behavior of flocculated MFT are assessed. The results were compared with ohginal untreated MFT , which confirmed that in case flocculants are properly mixed with MFT, they have a positive effect on the dewatenng rate of the MFT. However, the final volume of the consolidated flocculated MFT when exposed to atmospheric drying can be significantly larger than the untreated MFT. The results provide a useful reference for future pilot-scale tests and commercial implementation of the atmosphenc drying method on flocculated MFT.

The effects of flow regulation on freeze-up regime Peace River, Taylor to the Slave River

Authors Andres, D. D.
Year of Publication: 1996

Abstract:
This report has reviewed the processes by which an ice cover forms on large regulated and non- regulated rivers. Explicit equations and algorithms have been presented that quantify these processes. Work that had been undertaken previously on the Peace River was also described to provide a framework for the calibration of these algorithms for the Peace River in both its regulated and non-regulated condition. The significant theoretical advances that were made include the development of a procedure to forecast freeze-up on a non-regulated river and the derivation of a stability relationship that uses both air temperature and discharge to determine whether a juxtaposed or consolidated ice cover will form. The latter development is important to characterize the type of ice cover that will occur on the Peace River under regulated conditions. In addition, the hydraulic characteristics of the Peace River were evaluated for six distinct reaches between the Slave River and Taylor using the existing data base. The climatological characteristics of the basin were summarized, along with a description of the spatial and temporal variation in the flows for the periods before and after regulation. Prior to regulation, at flows of less than 1000 m3/s, the river cooled from a maximum annual water temperature of about 22°C to 0°C at the same rate as the declining air temperature Ice began to form in early November in most years, and an ice cover formed by multiple lodgements when the surface ice concentration neared 100% and the discharge decreased sufficiently to reduce the width of the flow by about 10%. A stable ice cover usually formed in early November at Peace Point and in late November or early December at Peace River. There is no data for Taylor, although the freeze-up probably occurred in early December. The ice thickness associated with this type of freeze-up generally ranged from 0.5 to 1.0 m. The stage increase was typically between 1.0 to 2.0 m. In some cases, due to declining flows during and following the formation of the ice cover, the stage decreased after the ice cover was established. Since regulation, the discharges are, on the average, about two to three times greater than those prior to regulation. This high discharge of relatively warm water from upstream of the has delayed the time of freeze-up and shortened ice duration of the ice cover significantly in the reaches upstream of Fort Vermilion. At Taylor, and upstream of the BC/Alberta border, an ice cover is an exception rather than a rule. At Peace River, and downstream to Fort Vermilion, the freeze-up date has been delayed by as much as one to two months. Only minor effects due to regulation are evident on the freeze-up ice regime downstream of the Vermilion Chutes and at Peace Point. After regulation, the ice cover downstream the Notikewin River generally forms by juxtaposition due to the very mild slopes. The ice cover thickness in these two reaches is only about 0.5 m thick, immediately after freeze-up and the stage increase associated with freeze-up is only about 1 to 2 m. The increase in stage is due mostly to the additional flow resistance of the ice cover. In the reaches between the Notikewin River and Dunvegan, where higher slopes are evident, either a juxtaposed or consolidated ice cover can form. For typical post-regulation discharges, the air temperature must be at least -30°C for a juxtaposed cover to form. To ensure that a juxtaposed ice cover forms, regardless of the air temperatures expected, the discharge should be less than 800 to 1000 m3/s. The stage increase under a juxtaposed ice cover is less than 2 m, but for a consolidatedice cover the stage increase can be as great as 5 ra, with an ice thickness of about 4 m. Between Hudson Hope and Dunvegan, the steeper river slopes prevent the formation of a juxtaposed ice cover for any reasonable combination of discharge and air temperature. Although the development of an ice cover in these two reaches is infrequent and when it does occur its duration is short lived, the formation thickness can approach 5 m and the increase in the stage can be up to 6 m. The main physical impacts on the environment relate primarily to (1) the existence of high water levels for long periods of time in areas where a consolidated ice cover has developed, (2) the losses in up to 30% of the flow into channel storage as the ice cover advances, (3) the potential unstable water levels and ice thicknesses that are evident within 100 km of the advancing ice cover, (4) the reduction in the duration of an ice cover for most of the length of the Peace River, and (5) dramatically thicker deposits of frazil in low velocity areas of the river upstream of the Vermilion Chutes. Although algorithms have been developed for many of the process identified on the Peace River, additional work is required to improve the modelling capabilities. Additional observations and measurements need to be carried out downstream of the Vermilion Chutes to characterize better the freeze-up process in that reach. It is also suggested that bench marks established around the Vermilion Chutes as part of this study be referenced to a common datum. This will improve the understanding of the hydraulics of the Chutes. From a modelling point of view, more work is required to verify the stability criteria used in determining the dominant mode of cover formation. An important component of this work will be the unsteady simulation of a consolidation event. Also, some effort must be expended to explicitly model the formation of frazil ice floes.

The effects of meso-scale topography on the performance of engineered soil covers

Authors Kelln, C. J.
Year of Publication: 2008

Abstract:
Understanding the hydrological controls on subsurface flow and transport is of considerable importance in the study of reclaimed landscapes in the oil sands region of Canada. A significant portion of the reclaimed landscape will be comprised of a thin veneer (~ 1 m) of clay-rich reclamation soil overlying saline-sodic shale overburden, which is a waste by-product from the mining process. The global objective of this study was to investigate the first-order controls on soil moisture and salt transport dynamics within clay-rich reclamation covers overlying low permeability waste substrates. The study site is located in a cold, semi-arid climate in the oil sands region of northern Alberta. Preferential flow was the dominant mechanism responsible for the development of perched water table conditions on the cover-waste interface during the spring snow melt. Hydrological and geochemical data indicated that snowmelt infiltration occurs via the macroporosity while the ground is still frozen. An isotope hydrograph separation conducted on water collected in a weeping tile confirmed the presence of fresh snowmelt water at the onset of subsurface flow. This water transitions to a chemical signature that is comprised of approximately 80% connate pore water as a result of chemical equilibration between pore water in the soil matrix and fresh water in the micropores. Detailed mapping of the spatial distribution of soil moisture and salts within a reclamation cover indicated the lower-slope positions are wetter due to the accumulation surface run-off and frozen ground infiltration in spring. Increased soil moisture conditions in lower-slope positions accelerate salt ingress, while drier conditions in middle and upper-slope positions attenuate salt ingress. The data indicated that fresh snowmelt water is bypassing the soil matrix higher in the cover profile. Subsurface flow and deep percolation are key mechanisms mitigating vertical salt ingress in lower and upper slope positions. The mesotopography of the cover-waste interface imposes a direct control on the depth of perched water and the downslope routing of water. Undulations in the cover-waste interface cause the depth of perched water to vary considerably (± 20– 60 cm) over short distances (< 5 m), while saturated subsurface flow is routed through the lowest elevations in the cover profile. A numerical analysis of subsurface flow was able to simulate both the discharge rate and cumulative volume of flow to a weeping tile. Composite hydraulic functions were used in the simulations to account for the increased hydraulic conductivity and drainable porosity created by the macroporosity at near-saturated conditions. The transient Na+ concentration of discharge water was modelled using the concept of an equivalent porous medium. The good match between measured and modelled data verified the conceptual model, which contends that saturated subsurface flow is dominated by the fracture network and that the concentration of discharge water is function of the depth of perched water. Finally, the results from this study suggest the mesotopography of the cover-waste interface could be used to manage „excess‟ water and salts within the landscape.

The effects of nutrient and peat amendments on oil sands reclamation wetlands: A microcosm study

Authors Chen, H. H.
Year of Publication: 2011

Abstract:
Oil sand mining operations in Alberta, Canada produce large quantities of process water and mature fine tailing (MFT) during the bitumen extraction process. Wet landscape reclamation is one of the reclamation strategies proposed to utilize process water and MFT in the creation of aquatic reclamation environments that are economically and environmentally acceptable. In the interest of utilizing nutrient enrichment and peat amendment to improve aquatic flora and fauna colonization in new oil sands aquatic reclamation, this microcosm study was designed to assess the phytoplankton and periphyton growth (summer 2008), as well as benthic invertebrate colonization (summer 2009). Peat amendment significantly increased the growth of phytoplankton and periphyton by providing sufficient nutrients (total nitrogen, total phosphorus and dissolved organic carbon) to the system. In reference wetland, benthic invertebrate colonization was significantly increased by utilizing sand as bottom substrate and decreased by MFT/Sand mixture as bottom substrate. In OSPM-affected wetland, benthic invertebrate colonization was not affected by utilizing MFT/Sand as bottom substrate. In comparison to OSPM-affected wetlands, reference wetland had larger number of benthic invertebrate families and higher total abundance. In this research, experimental microcosms were constructed in three reclamation wetlands with different types of reclamation materials as the bottom substrates (sand, MFT + sand) and amendments (nutrient and/or peat) added to optimize growing conditions for phytoplankton and periphyton, thus creating a biological detrital layer over unfavourable substrates to enhance benthic invertebrate colonization. The growth estimates of phytoplankton and periphyton on MFT + sand without amendment were low in comparison to the control (water only, no substrate). In comparison to sand, MFT + sand had higher growth estimates at OSPM-affected sites, but lower growth estimates at reference site. The growth estimates of phytoplankton and periphyton on MFT + sand were significantly increased with peat amendment. Nutrient (nitrogen and phosphorus) enrichment insignificantly improved the phytoplankton and periphyton growth. Peat amendments elevated the concentrations of nitrogen, phosphorus and dissolved organic carbon in the system and maintained these high concentrations throughout the experiment period. Nutrient enrichment only temporarily (less than 3 weeks) elevated nitrogen and phosphorus levels as the nutrients added were quickly utilized by the system. Benthic invertebrate colonization was assessed in the following year. Sand treatments had increased total abundance and numbers of families of benthic invertebrate compared to the mature sediments of the reference wetland. In oil sand process material (OSPM)-affected wetlands, sand treatments had slightly lower abundance and fewer numbers of families in comparison to the mature sediments. In comparison to sand treatments, MFT + sand treatments had decreased total abundance in the reference wetland but not in OSPM-affected wetlands that received MFT input during its construction. Peat amendment and nutrient enrichment had no impact on benthic invertebrate total abundance or composition.

The effects of oil sands process-affected waters and their associated constituents on fathead minnow (Pimephales promelas) reproductive physiology

Authors Kavanagh, R. J.
Year of Publication: 2012

Abstract:
As part of their reclamation plan, oil sands operators propose to transfer the mature fine tailings, which are a by-product of the oil sands extraction process, to open-pits and cap them with either a layer of surface water or oil sands process-affected waters (OSPW). These oil sands pit lakes are expected to develop habitats with productive capabilities comparable to natural lakes in the region. The studies presented in this thesis evaluate the potential impact of OSPW and its associated constituents [i.e. acid- extractable organics (e.g. naphthenic acids; NAs) and salts] on the reproductive physiology of adult fathead minnow (Pimephales promelas). Through 14-21 day fathead minnow reproduction assays it was demonstrated that aged OSPW can impair spawning, lower plasma sex steroid concentrations, and reduce male secondary sexual characteristics. The acid-extractable organics in OSPW were demonstrated to have an adverse effect on fathead minnow reproductive physiology. Other studies showed that the high salinity which characterizes OSPW also influences toxicity. When fathead minnows were exposed to the OSPW extract and 700 mg/l of NaHCO3, the NaHCO3 reduced the inhibitory effects of the extract on the numbers of reproductive tubercles and plasma testosterone levels by reducing the uptake of NAE to the fish. Embyro and larval bioassays also revealed that NaHCO3 reduces the acute toxic effects of the OSPW extract. An assessment of a wild population of fathead minnows inhabiting an OSPW pond determined that there were differences in the condition factor (CF), gonadosomatic indices (GSIs), liver somatic indices (LSIs), male secondary sexual characteristics, and 11-ketotestosterone concentrations in the fathead minnows from the OSPW pond relative to fish collected at reference sites. The opercula of fathead minnows from the OSPW pond also differed from those of reference fish and an examination of the gills revealed that were a number of proliferative and degenerative alterations relative to reference fish. Collectively, these studies demonstrate that aged OSPW has the potential to negatively affect the reproductive physiology of fathead minnows and suggest that aquatic habitats with high NAs concentrations (>10 mg/l) will have adverse effects on fish.

The effects of oil sands wetlands on wood frogs (Rana sylvatica)

Year of Publication: 2010

Abstract:
Extraction of crude oil from oil sand produces solid (sand) and liquid (water with suspended fine particles) tailings materials, called oil sands process-affected materials (OSPM). These waste materials are stored on the mine site due to a “zero discharge” policy and must be reclaimed when operations end. The liquid tailings materials are known to contain naphthenic acids and polycyclic aromatic hydrocarbons and have high pH and salinity. One method of reclamation is the “wet landscape” approach, which involves using oil sands tailings materials to form wetlands that would mimic natural wetland ecological function. This study investigated the effects of wetlands formed with oil sands tailings materials on the survival and growth of wood frog (Rana sylvatica) larvae. In spring 2007, in-situ caging studies were completed in 14 wetlands that were of four different classes; young or old, reference or reclaimed. Tadpole survival was different between types of wetlands, with young tailings-affected wetlands (≤7 years old) having 41.5%, 62.6%, and 54.7% higher tadpole mortality than old tailings-affected (>7 years old), young reference, and old reference wetlands, respectively. Since old wetlands created from OSPM showed effects on tadpoles similar to those of reference wetlands, which had markedly lower toxicity than young tailings-affected wetlands, we provide evidence that wetlands, at least 7 years old, can sustain amphibian life.

The effects of petroleum coke amendments on macrophytes and aquatic invertebrates in northern Alberta Canada constructed wetlands

Authors Baker, L. F.
Year of Publication: 2007

Abstract:
Oil-sands operators of Fort McMurray, Alberta produce six million t/y of petroleum coke. The use of coke to stabilize clay-dominated mine tailings in constructed wetlands is being studied in landscape reclamation. We studied 'in situ' effects of petroleum coke amendments placed in three wetlands constructed with different materials over two years. Coke amendments did not significantly increase concentrations of trace metals in sediment pore waters or associated biota in plots. Growth of resident macrophyte species was not prohibited by coke amendments. Coke plots in a reference wetland contained fewer stress-intolerant invertebrates, than reference plots, likely due to avoidance of coke substrates. Adding peat reduced 'Chara' cover and biomass in the reference wetland, but had no impact on plants or invertebrates in the wetlands with little organic content. Overall, local coke amendment effects were detected in a reference wetland but not in two wetlands constructed with other oil sands process materials.

The effects of pretreatment on nanofiltration and reverse osmosis membrane filtration for desalination of oil sands process-affected water

Year of Publication: 2011

Abstract:
Oil sands process-affected water (OSPW) is largely produced from the oil sands operational process and requires the removal of toxicants for reuse. Nanofiltration (NF) and reverse osmosis (RO) membrane applications can be used to remove salt ions from OSPW. However, membrane treatments of OSPW are impeded by membrane fouling due to suspended solids; therefore, feed water must be pretreated to manage fouling. This research investigated NF and RO pretreatment methods such as coagulation–flocculation–sedimentation (CFS), with and without coagulant and coagulant aids. The optimal concentration of coagulant was verified by examining water turbidity and membrane surface zeta potential. The results show that membrane permeability was enhanced by the addition of coagulant and coagulant aids. Organic and oily constituents in OSPW increased the negative charge and hydrophobicity of the membrane, decreasing membrane performance. The flux recovery ratio was used to measure the effects of physical and chemical washing. Efficient chemical cleaning was achieved with 1 mM acid; flux recovery was achieved to 81% after cleaning. This study reveals that the pretreatment improves desalination of OSPW for both NF and RO membranes.

The effects of salinity on naphthenic acid toxicity to yellow perch: Gill and liver histopathology

Year of Publication: 2006

Abstract:
Naphthenic acids (NAs) are naturally occurring saturated linear and cyclic carboxylic acids found in petroleum,including the bitumen contained in the Athabasca Oil Sands deposit in Alberta,Canada. The processing of these oil sands leads to elevated concentrations of NAs,as well as increased salinity from produced waters as a result of ions leaching from the ores,the process aids, and the water associated with the deeper aquifers. These changes can result in waters that challenge reclamation of impacted waters associated with oil sands development. Laboratory tests examined the effects of salinity on NA toxicity using local young-of-the- year yellow perch exposed to a commercially available mixture of NAs (CNA) and an NA mixture that was extracted from oil sands process-affected water (ENA),with and without the addition of sodium sulfate (Na 2SO4). Gill and liver histopathological changes were evaluated in the surviving fish after 3 weeks of exposure. At 6.8 mg/L ENA and 3.6 mg/L CNA,100% mortality was observed, both with and without the addition of salt. Exposure of yellow perch to 25% of the NA required to give an LC100 (0.9 mg/L CNA; 1.7 mg/L ENA) resulted in high levels of gill proliferative (epithelial,mucous,and chloride cell) changes,a response that was increased with the addition of 1 g/L salt (Na2SO4) for the ENA. The significance of these changes was a reduced gill surface area, which likely caused a reduction in both the transport of NAs within the fish and the exchange of vital respiratory gases. While the gills were affected,no liver alterations were identified following NA or NA+salt exposures. Differences in the chemical composition of the NAs tested may explain the differences in the lethality and histopathology of yellow perch.

The effects of sedimentation on the aquatic biota

Year of Publication: 1978

Abstract:
A review of the effects of sedimentation on aquatic biota is presented. The detrimental effects of increased suspended and settled sediments on fish, bottom invertebrates, and primary productivity are documented. It is shown that the upper tolerance level for suspended sediment is between 80-100 mg/l for fish, and as low as 10-15 mg/l for bottom invertebrates. Recovery of the aquatic biota from increased sedimentation is dependent on the severity of sediment additions and the discharge level of the rivers or streams. Recovery from short-term additions of sediment is usually complete within one year. The use of remote sensing and biomonitoring to locate sources of sedimentation is discussed. Remote sensing can generally be used to identify point sources of sedimentation, define flow patterns, choose sampling stations, interpret ground survey data, and maintain permanent records of changes in water quality. Biomonitoring can be used to monitor water quality, especially with regard to sedimentation, since alterations in the environment are reflected by the indigenous biota. The sedimentation characteristics of the Alberta Oil Sands Environmental Research Program (AOSERP) study area are presented and observations are made on the potential for erosion and sediment production. The AOSERP study area is divided into twelve hydrological zones and each zone is classified for erosion potential. The zones having a high erosion potential are: (1) lower Ells basin and eastern slopes of Birch Mountains (Zone 1); (2) tributaries immediately north of Fort McMurray (Zone 6); (3) Christina River basin (Zone 7); (4) Hangingstone and Horse River basins. (Zone 8); (Fort MacKay River basin (Zone 9); (6) Dunkirk River basin (Zone 10); and (7) upper Ells River basin (Zone 11). Road construction, pipeline construction, general construction (urban and industrial sites), vegetation removal, overburden removal, and pit excavation, tailing ponds, settling ponds, and diversion channels were identified as possible sources of unnatural increases of suspended and settled sediments in the AOSERP study area. The effect of development activities on the hydrological regime and the aquatic biota is shown. The scale of the disturbance and the length of the recovery period are also predicted. Development activities such as road building and pipeline construction will affect a number of the watershed basins; therefore, they were classified as having regional effect and were considered to be of greatest concern.

The effects of sodium chloride sodium sulfate and consolidated tailings water on jack pine (Pinus banksiana Lamb.) seedlings

Authors Franklin, J. A.
Year of Publication: 2002

Abstract:
The Athabasca Oil Sands deposit in Northern Alberta is the site of large-scale mining operations (Syncrude Canada Ltd. and Suncor Energy Inc.) that produce relatively saline tailings water, sand and fine tailings. Reclamation goals for the site of current mining operations include the establishment of a productive forest, however the salt tolerance of many native forest plants, including jack pine, is largely unknown. The objective of thesis research program was to determine the effects of water associated with composite tailings (CT water) and its main salts, NaCl and Na2SO4 on the growth and physiology of jack pine (Pinus banksiana Lamb.), and to investigate the mechanisms of salt injury and tolerance. Jack pine seedlings were grown in sand or solution culture, and exposed to solutions of NaCl, Na2SO4 or CT water in a series of experiments. Treatment of one-year-old seedlings with NaCl had a more detrimental effect than did isomolar Na2SO4 with respect to most of the parameters measured, suggesting that ion toxicity is the dominant effect. While Na2SO4-treated plants showed reduced tissue K +and Ca2+, of which deficiencies are often associated with salt stress, plants treated with NaCl had significantly increased levels of several nutritional elements. Shoot Na was greater in NaCl-treated plants than in plants treated with Na2SO4 at equivalent Na levels, and was correlated with tissue injury only in the former. Tissue electrolyte leakage was more closely related to treatment Cl level, than to Na level, suggesting that Cl is associated with increased membrane permeability. Transpiration rates were similar in all salt treatments, and the greater translocation of Na in NaCl-treated plants was the result of greater root permeability to Na. Seedlings treated with CT water exhibited similar growth inhibition and injury to plants treated with salts, and injury was related to both shoot Na and Cl levels. The uptake of mineral nutrients may be influenced by the high pH of the treatment solution. Because jack pine appears to be sensitive to substrate Cl, planting of this species is recommended only on sites where Cl is low.

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