The purpose of this project was to collect and prepare fish from 23 sites on the Peace, Athabasca and Slave River drainages for conducting physical, physiological, contaminant and biophysical analyses. Sites were located on the Peace, Smoky, Little Smoky, Wapiti, Wabasca, Athabasca, McLeod, Pembina, Lesser Slave and Clearwater Rivers. The fish species targeted for collection and analyses were burbot (primary target species), northern pike, longnose sucker and flathead chub. The sampling and handling protocols for the collection of fish tissues were designed to allow for a wide range of contaminant and biochemical analyses on an individual fish. There analyses included gross pathology, contaminant, metals (e.g., mercury), liver mixed function oxygenase induction, sex steroids and gonad morphology, retinols (Vitamin A), metallothioneins (proteins produced by exposure to heavy metals) and basic life history information.