Adverse effects of low environmental oxygen on fish reproduction represents a distinct possibility in the Athabasca and Peace drainages. Species spawning in late fall and winter, such as burbot, would be the most likely to be impacted by low dissolved oxygen (DO). This report describes a study whose objective was to define the temporal changes in reproductive hormones, identify changes in blood electrolytes, haemoglobin, hematocrit, and blood oxygen content, and evaluate the viability of eggs and sperm for burbot during the period of sexual development under normal and hypoxic conditions. The results evaluate the potential of realistic winter water temperatures and hypoxic conditions to impair reproductive performance and induce stress in spawning burbot in the Northern River Basins Study area.