Several oil sands mines and refineries are currently recovering oil from the Athabasca River region near Fort McMurray, Alberta. Planning and construction of additional oil sands mines are in various stages of completion. Due to environmental concerns for aquatic life in areas downstream from current and future oil sands activities, surveys were conducted between 1998 and 2000 to determine whether the quality of water and sediments in tributaries of the Athabasca River are affected by flowing through reaches with exposure to natural oil sand deposits. This paper presents the results for metals in bed and suspended sediments collected from the Mackay, Steepbank, and Ells rivers during those surveys. The suspended sediments had a clay content (n = 7) ranging from 36.2% to 65.0%, while bed sediments, which consisted of a predominant sand fraction, had percent clay (n = 39) ranging from 0.0% to 38.1%. A Mann–Kendall non-parametric analysis to assess the longitudinal trend of the metals in the bed sediments found no significant (α = 0.05) downstream trend in the Mackay or Steepbank rivers; however, the Ells River displayed a generally decreasing tend from upstream to downstream. The results provide no indication that metal concentrations in the bed sediments and/or suspended sediments of the Mackay, Steepbank, and Ells rivers increase significantly as the three tributaries flow through reaches that have natural oil sand exposures (McMurray Formation).