The objective of this project was to examine vitamin A and E indices in fish collected from the Peace, Athabasca and Slave rivers and their major tributaries in 1994, part of a multi-faceted study into altered fish physiological function as a result of environmental contaminants. The fish species targeted for collection and analyses were burbot (primary target species), northern pike and longnose sucker. Biochemical analyses were conducted in the laboratory to determine the levels of retinol, dehydroretinol, retinyl palmitate (three storage forms of vitamin A), B-carotene (precursor form of vitamin A), and tocopherol in the liver of each fish specimen. Additional retinyl palmitate analysis was performed on longnose suckers collected from the upper Athabasca River in the fall, 1992.