@proceedings {52930,
	title = {Molecular identification of a yellow perch viral disease associated with exposure to oil sands process affected waters (PO)},
	year = {2011},
	note = {IN: Proceedings of the 37th Annual Aquatic Toxicity Workshop October 3-62010 Toronto Ontario. Fletcher T. D. Holdway D. Simmons M. Dutton and L.E. Burridge (Eds). Fisheries and Oceans Canada St. Andrews New Brunswick. Canadian Technical Report of Fisheries},
	month = {10/2010},
	address = {Toronto, ON },
	abstract = {Large volumes of tailings and process affected water are generated as a result of oil sand mining processes. This presentation discussed the safe incorporation of these wastes into the terrestrial and aquatic landscape. A study was conducted in which yellow perch were stocked into experimental ponds, namely Demonstration Pond and South Bison Pond, during the periods of 1995-1997 and 2008-2010. Demonstration Pond was comprised of mature fine tailings capped with natural surface water, while South Bison Pond was formed at a site surrounded by overburden or lean oil sands. Disease surveys were conducted at these experimental ponds and also at Mildred, Sucker, and Kimowin Lakes. External white nodular lesions, characteristic of lymphocystis disease were observed on perch at all sites except Kimowin Lake. The identity of the virus was confirmed by DNA extraction and PCR with genotype generic major capsid protein gene primers. The presence of lymphocystis disease virus in perch was confirmed through sequencing of PCR results. The viral genotype appeared to be different from any previously isolated viral genotype. During the course of the study, there was an increasing incidence of the disease at Demonstration Pond and a decreasing incidence at the South Bison Pond. The intensity of the disease was found to be proportional to the incidence, which was positively correlated with changes in naphthenic acid concentration.},
	keywords = {field, fish, inventory, naphthenic acids, survey, tailings water, toxicity},
	author = {Hogan, N. and Van Den Heuvel, M. and Phalen, L.}
}
