@article {52218,
	title = {Endocrine disruption and oxidative stress in larvae of Chironomus dilutus following short-term exposure to fresh or aged oil sands process-affected water},
	volume = {142-143},
	year = {2013},
	month = {10/2013},
	pages = {7 pages },
	publisher = {Aquatic Toxicology },
	abstract = {Understanding the toxicity of oil sands process-affected water (OSPW) is a significant issue associated with the production of oil from the Alberta oil sands. OSPW is acutely and chronically toxic to organisms, including larvae of Chironomus dilutus. In this study, fresh OSPW ({\textquoteleft}WIP-OSPW{\textquoteright}) was collected from the West In-Pit settling pond and aged OSPW ({\textquoteleft}FE5-OSPW{\textquoteright}) was collected from the FE5 experimental reclamation pond, both of which are located on the Syncrude Canada Ltd. lease site near Fort McMurray, Alberta, Canada. Larvae of C. dilutus were exposed to a freshwater control, WIP-OSPW, or FE5-OSPW for 4 or 7 days and survival, growth, and markers of oxidative stress and endocrine disruption were assessed. Survival was not significantly different among treatment groups. Compared to masses of larvae exposed to freshwater, masses of larvae exposed to WIP-OSPW were 49\% lesser on day 4 and 62\% lesser on day 7. However, organisms exposed to FE5-OSPW did not have significantly lesser masses than controls. Abundances of transcripts of glutathione-s-transferase (gst), catalase (cat), and glutathione peroxidase (gpx), which are important for the response to oxidative stress, were significantly altered in larvae exposed to WIP-OSPW, but not FE5-OSPW, relative to controls. Peroxidation of lipids was greater in larvae exposed to WIP-OSPW, but not FE5-OSPW. Exposure to fresh OSPW might have caused endocrine disruption because abundances of transcripts of the steroid hormone receptors, ultraspiricle protein (usp), ecysteroid receptor (esr), and estrogen related receptor (err) were greater in larvae exposed to WIP-OSPW for 7 days, but not FE5-OSPW. These results suggest that lesser growth of larvae of C. dilutus exposed to fresh OSPW might be due to oxidative stress and disruption of endocrine processes, and that aging of OSPW attenuates these adverse effects.},
	keywords = {invertebrates, Syncrude, tailings water, toxicity, UofS},
	url = {http://www.usask.ca/toxicology/jgiesy/pdf/publications/JA-740.pdf},
	author = {Wiseman, S.B. and Anderson, J.C. and Liber, K. and Giesy, J. P.}
}
