@article {52057,
	title = {Differential changes in gene expression in rainbow trout hepatocytes exposed to extracts of oil sands process-affected water and the Athabasca River},
	volume = {155},
	year = {2012},
	month = {01/2012},
	pages = {8 pages  },
	publisher = {Comparative Biochemistry and Physiology Part C Toxicology \& Pharmacology },
	abstract = {The oil sands region of northern Alberta represents the world{\textquoteright}s largest reserves of bitumen, and the accelerated pace of industrial extraction activity has raised concern about the possible impacts on the Athabasca River and its tributaries. An ecotoxicogenomic study was undertaken on Oncorhynchus mykiss trout hepatocytes exposed to extracts of water samples near the oil sand development area, as well as to oil sands process-affected water (OSPW) extracts using the quantitative reverse transcriptase polymerase chain reaction technique. The expression of the following genes (mRNA) was monitored to track changes in xenobiotic biotransformation (CYP1A1, CYP3A4, glutathione S-transferase, multi-drug resistance transporter), estrogenicity (estrogen receptor and vitellogenin), oxidative stress (superoxide dismutase and metallothionein) and DNA repair activity (DNA ligase). The extent of DNA-aromatic hydrocarbon adducts was also determined in cells by immuno-staining. A comparative analysis of gene expression between the river/lake and OSPW samples revealed that CYP3A4, metallothioneins, DNA ligase and GST genes, were specifically expressed by OSPW. Cells exposed to OSPW, commercial naphthenic acids, and benzo(a)pyrene showed increased polyaromatic hydrocarbon DNA-adducts, as determined by cell immunofluorescence analysis. Other genes were induced by all types of water samples, although the induction potential was stronger in OSPW most of the time (e.g., VTG gene was expressed nearly 15-fold by surface waters from the lake and river samples but increased to a maximum of 31-fold in OSPW). A multivariate discriminant function analysis revealed that the lake and river water samples were well discriminated from the OSPW. The CYP3A4 gene was the most highly expressed gene in cells exposed to OSPW and responded less to the lake or river water in the Athabasca River area. This study identified a suite of gene targets that responded specifically to OSPW extracts, which could serve as toxicogenomic fingerprints of OSPW contamination. 

Differential changes in gene expression in rainbow trout hepatocytes exposed to extracts of oil sands process-affected water and the Athabasca River (PDF Download Available). Available from: https://www.researchgate.net/publication/221753631_Differential_changes_in_gene_expression_in_rainbow_trout_hepatocytes_exposed_to_extracts_of_oil_sands_process-affected_water_and_the_Athabasca_River [accessed Jan 18, 2016].},
	keywords = {analytical methodology, Athabasca River, federal government, fish, tailings water, toxicity, tributaries},
	url = {https://www.researchgate.net/publication/221753631},
	author = {Gagne, F. and Douville, M. and Andre, C. and Debenst, T. and Talbot, A. and Sherry, J. and Hewitt, L. M. and Frank, R. A. and McMaster, M. E. and Parrott, J. and Bickerton, G.}
}
